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hilyte 555 aβ 1–40  (AnaSpec)

 
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    AnaSpec hilyte 555 aβ 1–40
    Hilyte 555 Aβ 1–40, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a%CE%B2+(1%E2%80%9340)+hilyte/hilyte+fluor+647+beta+amyloid++1+42+/pm40277938-48-1-11
    Average 90 stars, based on 1 article reviews
    hilyte 555 aβ 1–40 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: A flow cytometry–based in vitro assay reveals that formation of apolipoprotein E (ApoE)–amyloid beta complexes depends on ApoE isoform and cell type
    Article Snippet: In the second version of the assay, synthetic Aβ (1 - 42) HiLyte Fluor 647 – labeled (#AS-64161, AnaSpec) or Aβ (1 - 40) HiLyte Fluor 647 – labeled (#AS-60493, AnaSpec) was reconstituted in 100ul DMSO to a stock concentration of 1ug/ul in low retention tubes, pipetted for 10min at room temperature, aliquoted and frozen overnight at - 20oC.

    Article Title: Extracellular Vesicle-Serpine-1 Affects Neural Progenitor Cell Mitochondrial Networks and Synaptic Density: Modulation by Amyloid Beta and HIV-1.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte 647 were purchased from Anaspec (San Jose, CA) and dissolved in PBS.

    Article Title: Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges
    Article Snippet: A solution of Aβ 1-40 HiLyte-555 (Anaspec) or10 kDa Cascade blue dextran (Thermofischer D1976) was backfilled into the tubing and the bevel of the needle was inserted into the cisterna magna.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: HIV-1 Transactivator Protein Induces ZO-1 and Neprilysin Dysfunction in Brain Endothelial Cells via the Ras Signaling Pathway.
    Article Snippet: Aβ (1–40) HiLyte (a fluorescently labelled Aβ) was purchased from AnaSpec (San Jose, CA, USA).

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Enzyme-linked Immunosorbent Assay:

    Article Title: A flow cytometry–based in vitro assay reveals that formation of apolipoprotein E (ApoE)–amyloid beta complexes depends on ApoE isoform and cell type
    Article Snippet: In the second version of the assay, synthetic Aβ (1 - 42) HiLyte Fluor 647 – labeled (#AS-64161, AnaSpec) or Aβ (1 - 40) HiLyte Fluor 647 – labeled (#AS-60493, AnaSpec) was reconstituted in 100ul DMSO to a stock concentration of 1ug/ul in low retention tubes, pipetted for 10min at room temperature, aliquoted and frozen overnight at - 20oC.

    Article Title: Extracellular Vesicle-Serpine-1 Affects Neural Progenitor Cell Mitochondrial Networks and Synaptic Density: Modulation by Amyloid Beta and HIV-1.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte 647 were purchased from Anaspec (San Jose, CA) and dissolved in PBS.

    Article Title: Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges
    Article Snippet: A solution of Aβ 1-40 HiLyte-555 (Anaspec) or10 kDa Cascade blue dextran (Thermofischer D1976) was backfilled into the tubing and the bevel of the needle was inserted into the cisterna magna.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: HIV-1 Transactivator Protein Induces ZO-1 and Neprilysin Dysfunction in Brain Endothelial Cells via the Ras Signaling Pathway.
    Article Snippet: Aβ (1–40) HiLyte (a fluorescently labelled Aβ) was purchased from AnaSpec (San Jose, CA, USA).

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    BIA-KA:

    Article Title: A flow cytometry–based in vitro assay reveals that formation of apolipoprotein E (ApoE)–amyloid beta complexes depends on ApoE isoform and cell type
    Article Snippet: In the second version of the assay, synthetic Aβ (1 - 42) HiLyte Fluor 647 – labeled (#AS-64161, AnaSpec) or Aβ (1 - 40) HiLyte Fluor 647 – labeled (#AS-60493, AnaSpec) was reconstituted in 100ul DMSO to a stock concentration of 1ug/ul in low retention tubes, pipetted for 10min at room temperature, aliquoted and frozen overnight at - 20oC.

    Article Title: Extracellular Vesicle-Serpine-1 Affects Neural Progenitor Cell Mitochondrial Networks and Synaptic Density: Modulation by Amyloid Beta and HIV-1.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte 647 were purchased from Anaspec (San Jose, CA) and dissolved in PBS.

    Article Title: Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges
    Article Snippet: A solution of Aβ 1-40 HiLyte-555 (Anaspec) or10 kDa Cascade blue dextran (Thermofischer D1976) was backfilled into the tubing and the bevel of the needle was inserted into the cisterna magna.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: HIV-1 Transactivator Protein Induces ZO-1 and Neprilysin Dysfunction in Brain Endothelial Cells via the Ras Signaling Pathway.
    Article Snippet: Aβ (1–40) HiLyte (a fluorescently labelled Aβ) was purchased from AnaSpec (San Jose, CA, USA).

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Derivative Assay:

    Article Title: A flow cytometry–based in vitro assay reveals that formation of apolipoprotein E (ApoE)–amyloid beta complexes depends on ApoE isoform and cell type
    Article Snippet: In the second version of the assay, synthetic Aβ (1 - 42) HiLyte Fluor 647 – labeled (#AS-64161, AnaSpec) or Aβ (1 - 40) HiLyte Fluor 647 – labeled (#AS-60493, AnaSpec) was reconstituted in 100ul DMSO to a stock concentration of 1ug/ul in low retention tubes, pipetted for 10min at room temperature, aliquoted and frozen overnight at - 20oC.

    Article Title: Extracellular Vesicle-Serpine-1 Affects Neural Progenitor Cell Mitochondrial Networks and Synaptic Density: Modulation by Amyloid Beta and HIV-1.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte 647 were purchased from Anaspec (San Jose, CA) and dissolved in PBS.

    Article Title: Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges
    Article Snippet: A solution of Aβ 1-40 HiLyte-555 (Anaspec) or10 kDa Cascade blue dextran (Thermofischer D1976) was backfilled into the tubing and the bevel of the needle was inserted into the cisterna magna.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: HIV-1 Transactivator Protein Induces ZO-1 and Neprilysin Dysfunction in Brain Endothelial Cells via the Ras Signaling Pathway.
    Article Snippet: Aβ (1–40) HiLyte (a fluorescently labelled Aβ) was purchased from AnaSpec (San Jose, CA, USA).

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Confocal Microscopy:

    Article Title: A flow cytometry–based in vitro assay reveals that formation of apolipoprotein E (ApoE)–amyloid beta complexes depends on ApoE isoform and cell type
    Article Snippet: In the second version of the assay, synthetic Aβ (1 - 42) HiLyte Fluor 647 – labeled (#AS-64161, AnaSpec) or Aβ (1 - 40) HiLyte Fluor 647 – labeled (#AS-60493, AnaSpec) was reconstituted in 100ul DMSO to a stock concentration of 1ug/ul in low retention tubes, pipetted for 10min at room temperature, aliquoted and frozen overnight at - 20oC.

    Article Title: Extracellular Vesicle-Serpine-1 Affects Neural Progenitor Cell Mitochondrial Networks and Synaptic Density: Modulation by Amyloid Beta and HIV-1.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte 647 were purchased from Anaspec (San Jose, CA) and dissolved in PBS.

    Article Title: Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges
    Article Snippet: A solution of Aβ 1-40 HiLyte-555 (Anaspec) or10 kDa Cascade blue dextran (Thermofischer D1976) was backfilled into the tubing and the bevel of the needle was inserted into the cisterna magna.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: HIV-1 Transactivator Protein Induces ZO-1 and Neprilysin Dysfunction in Brain Endothelial Cells via the Ras Signaling Pathway.
    Article Snippet: Aβ (1–40) HiLyte (a fluorescently labelled Aβ) was purchased from AnaSpec (San Jose, CA, USA).

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Staining:

    Article Title: A flow cytometry–based in vitro assay reveals that formation of apolipoprotein E (ApoE)–amyloid beta complexes depends on ApoE isoform and cell type
    Article Snippet: In the second version of the assay, synthetic Aβ (1 - 42) HiLyte Fluor 647 – labeled (#AS-64161, AnaSpec) or Aβ (1 - 40) HiLyte Fluor 647 – labeled (#AS-60493, AnaSpec) was reconstituted in 100ul DMSO to a stock concentration of 1ug/ul in low retention tubes, pipetted for 10min at room temperature, aliquoted and frozen overnight at - 20oC.

    Article Title: Extracellular Vesicle-Serpine-1 Affects Neural Progenitor Cell Mitochondrial Networks and Synaptic Density: Modulation by Amyloid Beta and HIV-1.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte 647 were purchased from Anaspec (San Jose, CA) and dissolved in PBS.

    Article Title: Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges
    Article Snippet: A solution of Aβ 1-40 HiLyte-555 (Anaspec) or10 kDa Cascade blue dextran (Thermofischer D1976) was backfilled into the tubing and the bevel of the needle was inserted into the cisterna magna.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: HIV-1 Transactivator Protein Induces ZO-1 and Neprilysin Dysfunction in Brain Endothelial Cells via the Ras Signaling Pathway.
    Article Snippet: Aβ (1–40) HiLyte (a fluorescently labelled Aβ) was purchased from AnaSpec (San Jose, CA, USA).

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Fluorescence:

    Article Title: A flow cytometry–based in vitro assay reveals that formation of apolipoprotein E (ApoE)–amyloid beta complexes depends on ApoE isoform and cell type
    Article Snippet: In the second version of the assay, synthetic Aβ (1 - 42) HiLyte Fluor 647 – labeled (#AS-64161, AnaSpec) or Aβ (1 - 40) HiLyte Fluor 647 – labeled (#AS-60493, AnaSpec) was reconstituted in 100ul DMSO to a stock concentration of 1ug/ul in low retention tubes, pipetted for 10min at room temperature, aliquoted and frozen overnight at - 20oC.

    Article Title: Extracellular Vesicle-Serpine-1 Affects Neural Progenitor Cell Mitochondrial Networks and Synaptic Density: Modulation by Amyloid Beta and HIV-1.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte 647 were purchased from Anaspec (San Jose, CA) and dissolved in PBS.

    Article Title: Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges
    Article Snippet: A solution of Aβ 1-40 HiLyte-555 (Anaspec) or10 kDa Cascade blue dextran (Thermofischer D1976) was backfilled into the tubing and the bevel of the needle was inserted into the cisterna magna.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: HIV-1 Transactivator Protein Induces ZO-1 and Neprilysin Dysfunction in Brain Endothelial Cells via the Ras Signaling Pathway.
    Article Snippet: Aβ (1–40) HiLyte (a fluorescently labelled Aβ) was purchased from AnaSpec (San Jose, CA, USA).

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Labeling:

    Article Title: A flow cytometry–based in vitro assay reveals that formation of apolipoprotein E (ApoE)–amyloid beta complexes depends on ApoE isoform and cell type
    Article Snippet: In the second version of the assay, synthetic Aβ (1 - 42) HiLyte Fluor 647 – labeled (#AS-64161, AnaSpec) or Aβ (1 - 40) HiLyte Fluor 647 – labeled (#AS-60493, AnaSpec) was reconstituted in 100ul DMSO to a stock concentration of 1ug/ul in low retention tubes, pipetted for 10min at room temperature, aliquoted and frozen overnight at - 20oC.

    Article Title: Extracellular Vesicle-Serpine-1 Affects Neural Progenitor Cell Mitochondrial Networks and Synaptic Density: Modulation by Amyloid Beta and HIV-1.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte 647 were purchased from Anaspec (San Jose, CA) and dissolved in PBS.

    Article Title: Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges
    Article Snippet: A solution of Aβ 1-40 HiLyte-555 (Anaspec) or10 kDa Cascade blue dextran (Thermofischer D1976) was backfilled into the tubing and the bevel of the needle was inserted into the cisterna magna.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: HIV-1 Transactivator Protein Induces ZO-1 and Neprilysin Dysfunction in Brain Endothelial Cells via the Ras Signaling Pathway.
    Article Snippet: Aβ (1–40) HiLyte (a fluorescently labelled Aβ) was purchased from AnaSpec (San Jose, CA, USA).

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Concentration Assay:

    Article Title: A flow cytometry–based in vitro assay reveals that formation of apolipoprotein E (ApoE)–amyloid beta complexes depends on ApoE isoform and cell type
    Article Snippet: In the second version of the assay, synthetic Aβ (1 - 42) HiLyte Fluor 647 – labeled (#AS-64161, AnaSpec) or Aβ (1 - 40) HiLyte Fluor 647 – labeled (#AS-60493, AnaSpec) was reconstituted in 100ul DMSO to a stock concentration of 1ug/ul in low retention tubes, pipetted for 10min at room temperature, aliquoted and frozen overnight at - 20oC.

    Article Title: Extracellular Vesicle-Serpine-1 Affects Neural Progenitor Cell Mitochondrial Networks and Synaptic Density: Modulation by Amyloid Beta and HIV-1.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte 647 were purchased from Anaspec (San Jose, CA) and dissolved in PBS.

    Article Title: Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges
    Article Snippet: A solution of Aβ 1-40 HiLyte-555 (Anaspec) or10 kDa Cascade blue dextran (Thermofischer D1976) was backfilled into the tubing and the bevel of the needle was inserted into the cisterna magna.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection.
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: HIV-1 Transactivator Protein Induces ZO-1 and Neprilysin Dysfunction in Brain Endothelial Cells via the Ras Signaling Pathway.
    Article Snippet: Aβ (1–40) HiLyte (a fluorescently labelled Aβ) was purchased from AnaSpec (San Jose, CA, USA).

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.

    Article Title: Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
    Article Snippet: Aβ (1–40) and Aβ (1–40) HiLyte were purchased from Anaspec (San Jose, CA) and Aβ (1–40) was dissolved in PBS.



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    90
    AnaSpec hilyte tm fluor 488-labeled β-amyloid (1–40) hf-488-aβ 1–40
    Fibril formation of Aβ 1–40 . ( A ). SDS–PAGE of Aβ 1–40 solution immediately after being prepared with silver staining on ice ( a ) or solution after incubation for 144 h ( b ). Fresh sample ( a ) showed strong bands at MW 10–16 kD, suggesting a low number of oligomers, especially monomers, dimers, and trimers, whereas incubated sample ( b ) showed a strong band at MW 250 kD or higher. TEM of the fresh solution showed granular structures of 9.0–13.8 nm in diameter ( B ), whereas that of the incubated solution showed a fibrous structure of approximately 25 nm in diameter ( C ). Scale bar: ( B ) 100 nm; ( C ) 500 nm.
    Hilyte Tm Fluor 488 Labeled β Amyloid (1–40) Hf 488 Aβ 1–40, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fibril formation of Aβ 1–40 . ( A ). SDS–PAGE of Aβ 1–40 solution immediately after being prepared with silver staining on ice ( a ) or solution after incubation for 144 h ( b ). Fresh sample ( a ) showed strong bands at MW 10–16 kD, suggesting a low number of oligomers, especially monomers, dimers, and trimers, whereas incubated sample ( b ) showed a strong band at MW 250 kD or higher. TEM of the fresh solution showed granular structures of 9.0–13.8 nm in diameter ( B ), whereas that of the incubated solution showed a fibrous structure of approximately 25 nm in diameter ( C ). Scale bar: ( B ) 100 nm; ( C ) 500 nm.

    Journal: International Journal of Molecular Sciences

    Article Title: In Vivo Dynamic Movement of Polymerized Amyloid β in the Perivascular Space of the Cerebral Cortex in Mice

    doi: 10.3390/ijms23126422

    Figure Lengend Snippet: Fibril formation of Aβ 1–40 . ( A ). SDS–PAGE of Aβ 1–40 solution immediately after being prepared with silver staining on ice ( a ) or solution after incubation for 144 h ( b ). Fresh sample ( a ) showed strong bands at MW 10–16 kD, suggesting a low number of oligomers, especially monomers, dimers, and trimers, whereas incubated sample ( b ) showed a strong band at MW 250 kD or higher. TEM of the fresh solution showed granular structures of 9.0–13.8 nm in diameter ( B ), whereas that of the incubated solution showed a fibrous structure of approximately 25 nm in diameter ( C ). Scale bar: ( B ) 100 nm; ( C ) 500 nm.

    Article Snippet: HiLyte Fluor 647-labeled Aβ 1–40 was obtained from AnaSpec (San Jose, CA, USA) and kept frozen at −20 °C.

    Techniques: SDS Page, Silver Staining, Incubation

    Distribution of tracers transported from the cortical surface. ( A , B ): The Hilyte Fluor 647-labeled Aβ 1–40 oligomer was found around a penetrating artery ( A ( a )), a penetrating vein ( A ( b )), and a capillary ( A ( c )) in a Tie2-GFP mouse. ( B ( a – c )) are enlarged images of red squares ( A ( a – c )), respectively. Notably, Aβ accumulated at only part of the vessel wall, not at the entire circumference, in all vessels observed. ( D ) enlarged from a red square in ( C ): FITC-dextran of 40 kD distributed over the entire circumference of a penetrating artery as well as in the parenchyma ( D ( b )). A double barrel was also observed (arrowhead). In contrast, the accumulation of Aβ oligomer was localized to part of the circumference ( D ( c )), which is evident in a merged image ( D ( a )). ( F ) enlarged from a red square in ( E ): Massive accumulation of Aβ fibrils was noticed at a penetrating vein ( F ( c )), whereas 40 kD dextran was distributed more widely ( F ( a , b )), a merged image of ( F ( b , c )). Scale bar: ( A , C , E ) 100 μm; ( B , D , F ) 10 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: In Vivo Dynamic Movement of Polymerized Amyloid β in the Perivascular Space of the Cerebral Cortex in Mice

    doi: 10.3390/ijms23126422

    Figure Lengend Snippet: Distribution of tracers transported from the cortical surface. ( A , B ): The Hilyte Fluor 647-labeled Aβ 1–40 oligomer was found around a penetrating artery ( A ( a )), a penetrating vein ( A ( b )), and a capillary ( A ( c )) in a Tie2-GFP mouse. ( B ( a – c )) are enlarged images of red squares ( A ( a – c )), respectively. Notably, Aβ accumulated at only part of the vessel wall, not at the entire circumference, in all vessels observed. ( D ) enlarged from a red square in ( C ): FITC-dextran of 40 kD distributed over the entire circumference of a penetrating artery as well as in the parenchyma ( D ( b )). A double barrel was also observed (arrowhead). In contrast, the accumulation of Aβ oligomer was localized to part of the circumference ( D ( c )), which is evident in a merged image ( D ( a )). ( F ) enlarged from a red square in ( E ): Massive accumulation of Aβ fibrils was noticed at a penetrating vein ( F ( c )), whereas 40 kD dextran was distributed more widely ( F ( a , b )), a merged image of ( F ( b , c )). Scale bar: ( A , C , E ) 100 μm; ( B , D , F ) 10 μm.

    Article Snippet: HiLyte Fluor 647-labeled Aβ 1–40 was obtained from AnaSpec (San Jose, CA, USA) and kept frozen at −20 °C.

    Techniques: Labeling

    Dynamic perivascular transportation of HiLyte Fluor 647-labeled Aβ 1–40 oligomers ( A , B ) and fibrils ( C , D ). The perivascular distribution of Aβ was captured in horizontal planes ( B , D ) and reconstructed in 3 dimensions every 30 min ( A , C ). FITC-labeled dextran at 40 kD was distributed in the entire circumference of penetrating vessels, whereas Aβ accumulated in only localized perivascular spaces ( B , D ). The accumulated mass of Aβ oligomers moved around the vessel wall and separated ( A ). Aβ fibrils formed a large mass and elongated in the direction of a penetrating vessel ( C ). Scale bar: ( B , D ) 10 μm. Three-dimensional images and dynamic motion are shown in .

    Journal: International Journal of Molecular Sciences

    Article Title: In Vivo Dynamic Movement of Polymerized Amyloid β in the Perivascular Space of the Cerebral Cortex in Mice

    doi: 10.3390/ijms23126422

    Figure Lengend Snippet: Dynamic perivascular transportation of HiLyte Fluor 647-labeled Aβ 1–40 oligomers ( A , B ) and fibrils ( C , D ). The perivascular distribution of Aβ was captured in horizontal planes ( B , D ) and reconstructed in 3 dimensions every 30 min ( A , C ). FITC-labeled dextran at 40 kD was distributed in the entire circumference of penetrating vessels, whereas Aβ accumulated in only localized perivascular spaces ( B , D ). The accumulated mass of Aβ oligomers moved around the vessel wall and separated ( A ). Aβ fibrils formed a large mass and elongated in the direction of a penetrating vessel ( C ). Scale bar: ( B , D ) 10 μm. Three-dimensional images and dynamic motion are shown in .

    Article Snippet: HiLyte Fluor 647-labeled Aβ 1–40 was obtained from AnaSpec (San Jose, CA, USA) and kept frozen at −20 °C.

    Techniques: Labeling